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KMID : 0381120080300040329
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Genes and Genomics
2008 Volume.30 No. 4 p.329 ~ p.336
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Confirmation of Satt228 Marker Tightly Linked to the Ti Locus in Four Different Soybean Populations
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Kim Myung-Sik
Huh Moo-Ryong
Jeong Woo-Hyeun
Park Mo-Se
Lee Kyoung-Ja
Shim Sang-In
Kim Min-Chul
Jung Woo-Suk
Lee Jai-Heon
Chung Jong-Il
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Abstract
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Soybean (Glycine max L.) Kunitz trypsin inhibitor (KTI) protein is an antinutritional factor. The objective of this research was to confirm a SSR marker tightly linked to the Ti locus in four different F5 populations. Four female parents (Glycine max L. cv. Jinpumkong2, Hannamkong, GS06, 20M183) of TiTi (KTI protein present) genotype and one male parent C242 of titi (KTI protein absent) genotype were used. Four different populations of F2 plants free of KTI protein were advanced to the F5 generation. Satt228 marker analysis showed the four female parents had the allele 1 (AA genotype) while the C242 male parent has the allele 2 (BB genotype). In seed, four parents had KTI protein and C242 had not KTI protein of 21.5 kDa. A total of 391 F5 plants derived from the four crosses (273 plants from Jinpunkong2 × C242, 17 plants from Hannamkong × C242, 45 plants from GS06 × C242, and 56 plants from 20M183 × C242) all have the allele 2 (BB genotype) for Satt228 marker. The 391 individual F6 seeds harvested from same individual F5 plants are also absent of the KTI protein. Complete cosegregation between the Satt228 marker allele and the Ti locus was observed in these four different populations.
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KEYWORD
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Kunitz trypsin inhibitor, molecular marker, soybean, Ti locus, marker confirmation, MAS
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